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JoVE Journal Bioengineering
Semiautomated Longitudinal Microcomputed Tomography-based Quantitative Structural Analysis of a Nude Rat Osteoporosis-related Vertebral Fracture Model
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Semiautomated Longitudinal Microcomputed Tomography-based Quantitative Structural Analysis of a Nude Rat Osteoporosis-related Vertebral Fracture Model

Article DOI: 10.3791/55928-v • 07:12 min • September 28th, 2017
September 28th, 2017 •
1, 1, 2,3,4, 1,2,3,5, 1,2,3,4,5, 1,2,3,4
1Skeletal Biotech Laboratory, Hebrew University-Hadassah Faculty of Dental Medicine, 2Department of Surgery, Cedars-Sinai Medical Center, 3Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, 4Biomedical Imaging Research Institute, Cedars-Sinai Medical Center, 5Department of Orthopedics, Cedars-Sinai Medical Center

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Summary
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The goal of this protocol is to generate a nude rat osteoporosis-related vertebral compression fracture model that can be longitudinally evaluated in vivo using a semiautomated microcomputed tomography-based quantitative structural analysis.

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Semiautomated Longitudinal Microcomputed Tomography-based Quantitative Structural Analysis Nude Rat Osteoporosis-related Vertebral Fracture Model Surgical Model Image Analysis Method Treatment Preclinical Vertebral Compression Fracture Glial Regenerative Medicine Effective Treatment Bone Regeneration Advantage Technique Accurate User Dependent Immune Rejection Concerns Bone Regeneration Anesthesia Isoflurane Nose Cone Maintenance Abdominal Area Electric Shaver Iodine-based Antiseptic Chlorhexidine Gluconate Ethanol Vet Ointment Dryness Toe Pinch Stimulus
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