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JoVE Journal Genetics
The Replica Set Method
Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
Journal (Genetics)
Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
Journal (Genetics)
Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
 

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

Article DOI: 10.3791/57819-v • 11:58 min • June 29th, 2018
June 29th, 2018 •
1, 1, 2,3, 4, 1
1Department of Biomedical Genetics, University of Rochester Medical Center, 2Department of Biostatistics and Computational Biology, University of Rochester Medical Center, 3Non-Clinical Statistics, Bristol-Myers Squibb, 4Department of Microbiology and Immunology, University of Rochester Medical Center

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Here we describe the Replica Set method, an approach to quantitatively measure C. elegans lifespan/survival and healthspan in a high-throughput and robust manner, thus allowing screening of many conditions without sacrificing data quality. This protocol details the strategy and provides a software tool for analysis of Replica Set data.

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Replica Set Method High-throughput Approach Caenorhabditis Elegans Lifespan Genetic Inter-relationships Aging Field Throughput Increase Quantitatively Measured Lifespan Data Software Analysis Age-related Phenotypes Stress Resistance Health Plan Changes RNAi Sub-library 96-well Plate LB With Ampicillin 96-pin Plate Replicator Bleach Sterilization Ethanol Dip Flame Tips Glycerol Stock Library Plate
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