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In JoVE (1)
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Articles by Shawn M. Gage in JoVE
JoVE Bioengineering
עצמיים אבי האנדותל Cell-מתזמן טכנולוגיה בדיקה biocompatibility עבור התקנים לב וכלי דם במודל חיה גדולה
1Department of Biomedical Engineering, Duke University, 2School of Medicine, Duke University, 3Department of Surgery, Duke University Medical Center, 4School of Medicine, University of Pennsylvania
שיטה טיטניום זריעת דם biomaterials ליצור קשר עם תאים עצמיים ו biocompatibility בדיקות מתואר. שיטה זו משתמשת ובתאים אנדותל צינורות טיטניום, זורעים בתוך דקות של ניתוח ההשתלה לתוך חזירי venae cavae. טכניקה זו ניתנת להתאמה מכשירים רבים אחרים ביו מושתלת.
Other articles by Shawn M. Gage on PubMed
Salvaging Vascular Access and Treatment of Severe Limb Edema: Case Reports on the Novel Use of the Hemodialysis Reliable Outflow Vascular Access Device
We report two cases in which patients on chronic hemodialysis presented with morbid unilateral edema of the upper extremity and chest on the side of a currently functioning arteriovenous access. Both patients were known to the vascular surgery service and had previously undergone multiple attempts to create and maintain vascular access. Both severe and disabling edema and the need to maintain dialysis access were of significant concern. These patients were taken to the operating room to address both issues. Upper extremity venography with central venous runoff revealed central vein stenosis and/or occlusion resistant to angioplasty and stenting. Ultimately, these two patients received the Hemodialysis Reliable Outflow vascular access device. The experiences in this study demonstrate the novel use of a relatively new vascular access device for salvage of a malfunctioning arteriovenous fistula or graft as well as treatment of symptoms and complications resulting from long standing vascular access.
Use of Autologous Blood-derived Endothelial Progenitor Cells at Point-of-care to Protect Against Implant Thrombosis in a Large Animal Model
Titanium (Ti) is commonly utilized in many cardiovascular devices, e.g. as a component of Nitinol stents, intra- and extracorporeal mechanical circulatory assist devices, but is associated with the risk of thromboemboli formation. We propose to solve this problem by lining the Ti blood-contacting surfaces with autologous peripheral blood-derived late outgrowth endothelial progenitor cells (EPCs) after having previously demonstrated that these EPCs adhere to and grow on Ti under physiological shear stresses and functionally adapt to their environment under flow conditions ex vivo. Autologous fluorescently-labeled porcine EPCs were seeded at the point-of-care in the operating room onto Ti tubes for 30 min and implanted into the pro-thrombotic environment of the inferior vena cava of swine (n = 8). After 3 days, Ti tubes were explanted, disassembled, and the blood-contacting surface was imaged. A blinded analysis found all 4 cell-seeded implants to be free of clot, whereas 4 controls without EPCs were either entirely occluded or partially thrombosed. Pre-labeled EPCs had spread and were present on all 4 cell-seeded implants while no endothelial cells were observed on control implants. These results suggest that late outgrowth autologous EPCs represent a promising source of lining Ti implants to reduce thrombosis in vivo.
Analysis of Infection Risk Following Covered Stent Exclusion of Pseudoaneurysms in Prosthetic Arteriovenous Hemodialysis Access Grafts
To determine whether exclusion of pseudoaneurysms with the use of a covered stent in prosthetic arteriovenous (AV) hemodialysis access grafts impacts the incidence of eventual AV graft infection.
