Articles by Shravan R. Kumar in JoVE

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

JoVE 3410 1/27/2012

Elvira Hukasova, Helena Silva Cascales, Shravan R. Kumar, Arne Lindqvist

Department of Cell and Molecular Biology, Karolinska Institutet

FRET-based reporters are increasingly used to monitor kinase and phosphatase activities in live cells. Here we describe a method on how to use FRET-based reporters to assess cell cycle-dependent changes in target phosphorylation.