Articles by Tara M. Regan in JoVE

The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds

JoVE 2389 2/15/2011

Michelle K. Leach¹, Zhang-Qi Feng², Caitlyn C. Gertz³, Samuel J. Tuck³, Tara M. Regan³, Youssef Naim³, Andrea M. Vincent³, Joseph M. Corey^1,3,4

¹Department of Biomedical Engineering, University of Michigan, ²State Key Laboratory of Bioelectronics, Southeast University, ³Department of Neurology, University of Michigan, ⁴Geriatric Research, Education and Clinical Center, Veterans Affairs Ann Arbor Health System

Aligned electrospun fibers direct the growth of neurons in vitro and are a potential component of nerve regeneration scaffolds. We describe a procedure for preparing electrospun fiber substrates and the serum-free culture of primary rat E15 sensory (DRG) and motor neurons. Visualization of neurons by immunocytochemistry is also included.