Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

June 23rd, 2023

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Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen^3,4, Kaelyn Sumigray^1,5,6

¹Department of Genetics, Yale School of Medicine, ²Department of Pediatrics, Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology, Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology, Yale School of Medicine, ⁵Yale Stem Cell Center, Yale School of Medicine, ⁶Yale Cancer Center, Yale School of Medicine

* These authors contributed equally

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Hier presenteren we een protocol om darmorganoïden van ratten te genereren en deze te gebruiken in verschillende stroomafwaartse toepassingen. Ratten zijn vaak een geprefereerd preklinisch model, en het robuuste intestinale organoïdesysteem voorziet in de behoefte aan een in vitro systeem om in vivo studies te begeleiden.

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Rat Intestinal Crypts Isolation and Intestinal Organoids Generation

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