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JoVE Immunology and Infection
JoVE Immunology and Infection includes content from a variety of fields working toward a shared initiative of improving global health. Techniques evaluating the biological response to pathogens from the molecular to organismal level are presented as well as therapeutic agents and their efficacy in treating disease.
 JoVE Immunology and Infection

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

1Department of Pathology, University of California, San Diego


JoVE 52257

This article describes a method for the generation and propagation of human T cell clones that specifically respond to a defined alloantigen. This protocol can be adapted for cloning human T cells specific for a variety of peptide-MHC ligands.

 JoVE Immunology and Infection

High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability

1Department of Medicine - Division of Infectious Diseases, Vanderbilt University School of Medicine, 2Tennessee Valley Healthcare Systems, U. S. Dept. of Veterans Affairs


JoVE 52122

Here we describe a method to visualize the oncogenic bacterial organelle known as the Cag Type IV Secretion System (Cag-T4SS). We find that the Cag-T4SS is differentially produced on the surface of H. pylori in response to varying conditions of iron availability.

 JoVE Immunology and Infection

A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium

1Department of Immunology, Genetics and Pathology, Uppsala University


JoVE 52112

The accessibility of reliable models to investigate vascular blood interactions in humans is lacking. We present an in vitro model of cultured primary human endothelial cells combined with human whole blood to investigate cellular interactions both in the blood (ELISA) and the vascular compartment (microscopy).

 JoVE Immunology and Infection

Proteomic Profiling of Macrophages by 2D Electrophoresis

1Inserm UMR744, University Lille Nord de France


JoVE 52219

Macrophages are the key cells involved in host pathogenicity. Macrophages display phenotypic and functional diversity that can be analysed and detected by proteomic analysis. This article describes how to perform 2D electrophoresis of primary cultures of human macrophages differentiated into M1 or M2 phenotype.

 JoVE Immunology and Infection

Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems

1National Center for Biodefense and Infectious Diseases, George Mason University


JoVE 52065

Plaque assays are the gold standard for viral quantification, utilizing entrapping overlays on host cellular monolayers to determine viral titers. While various semisolid overlays have traditionally been used, here we demonstrate plaque techniques comparing semisolid overlays to a novel liquid microcrystalline cellulose among several families of viruses.

 JoVE Immunology and Infection

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

1Integrated Department of Immunology and Integrated Center for Genes, Environment, and Health, National Jewish Health and University of Colorado School of Medicine


JoVE 51306

In this protocol, we describe methods to efficiently transfect murine macrophage cell lines with siRNAs using the Amaxa Nucleofector 96-well Shuttle System, stimulate the macrophages with lipopolysaccharide, and monitor the effects on inflammatory cytokine production.

 JoVE Immunology and Infection

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

1Biological Department, King Abdulaziz University, 2The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield


JoVE 52222

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

 JoVE Immunology and Infection

Pseudomonas aeruginosa Induced Lung Injury Model

1Department of Pharmacology, University of Illinois College of Medicine, University of Illinois at Chicago, 2Section of Pulmonary and Critical Care Medicine, Atlanta VAMC, Emory University, 3Biologic Resources Lab, University of Illinois at Chicago


JoVE 52044

We have developed a mouse lung injury model by intra-tracheal injection of bacteria Pseudomonas aeruginosa. This model mimics lung injury during pneumonia and is clinically relevant.

 JoVE Immunology and Infection

Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat

1School of Pharmacy, University College London, 2Mucin Biology Group, University of Gothenburg


JoVE 52018

Here, a procedure is described for the establishment of systemic infection in the neonatal rat with cultures of Escherichia coli K1. This non-invasive procedure permits colonization of the gastrointestinal tract, translocation of the pathogen to the systemic circulation, and invasion of the central nervous system at the choroid plexus.

 JoVE Immunology and Infection

Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy

1Department of Microbiology and Immunology, Albert Einstein College of Medicine


JoVE 52084

We describe how to visualize macrophage-C. neoformans (Cn) interactions in real time, with specific emphasis on the process of non-lytic exocytosis using digital light microscopy. Using this technique individually infected macrophages can be studied to ascertain various aspects of this phenomenon.

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