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Base Composition: The relative amounts of the Purines and Pyrimidines in a nucleic acid.
 JoVE Biology

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

1Department of Biology, California Institute of Technology, 2Department of Bioengineering, California Institute of Technology, 3Synthetic Biology Center, Department of Bioengineering, Massachusetts Institute of Technology, 4School of Physics and Astronomy, University of Minnesota


JoVE 50762

 JoVE Biology

Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation

1Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, 2A*STAR-Duke-NUS Neuroscience Research Partnership, Singapore, 3Department of Biochemistry, National University of Singapore, Singapore


JoVE 3770

 Science Education: Essentials of Organic Chemistry

Fractional Distillation

JoVE Science Education

Source: Laboratory of Dr. Nicolas Leadbeater — University of Connecticut 

Distillation is perhaps the most common laboratory technique employed by chemists for the purification of organic liquids. Compounds in a mixture with different boiling points separate into individual components when the mixture is carefully distilled. The two main types of distillation are "simple distillation" and "fractional distillation", and both are widely used in organic chemistry laboratories. Simple distillation is used when the liquid is (a) relatively pure (containing no more than 10% liquid contaminants), (b) has a non-volatile component, such as a solid contaminant, or (c) is mixed with another liquid with a boiling point that differs by at least 25 °C. Fractional distillation is used when separating mixtures of liquids whose boiling points are more similar (separated by less than 25 °C). This video will detail the fractional distillation of a mixture of two common organic solvents, cyclohexane and toluene.

 JoVE Bioengineering

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

1Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, 2The Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, 3Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine, University of Hong Kong


JoVE 53447

 JoVE Neuroscience

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

1Department of Cellular and Molecular Physiology, Yale University School of Medicine, 2Nanobiology Institute, Yale University, 3Department of Molecular Biophysics and Biochemistry, Yale University, 4Laboratoire de Neurophotonique, Université Paris Descartes, Faculté des Sciences Fondamentales et Biomédicales, Centre National de la Recherche Scientifique (CNRS)


JoVE 54349

 JoVE Bioengineering

Nanomanipulation of Single RNA Molecules by Optical Tweezers

1Nanoscale Engineering Graduate Program, College of Nanoscale Science and Engineering, University at Albany, State University of New York, 2Nanoscale Science Undergraduate Program, College of Nanoscale Science and Engineering, University at Albany, State University of New York, 3Nanobioscience Constellation, College of Nanoscale Science and Engineering, University at Albany, State University of New York, 4The RNA Institute, University at Albany, State University of New York, 5Department of Biological Sciences, University at Albany, State University of New York


JoVE 51542

 Science Education: Essentials of General Chemistry

Determining the Mass Percent Composition in an Aqueous Solution

JoVE Science Education

Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry

Determining the composition of a solution is an important analytical and forensic technique. When solutions are made with water, they are referred to as being aqueous, or containing water. The primary component of a solution is referred to as the solvent, and the dissolved minor component is called the solute. The solute is dissolved in the solvent to make a solution. Water is the most common solvent in everyday life, as well as nearly all biological systems. In chemistry labs, the solvent may be another liquid, like acetone, ether, or an alcohol. The solute can be a liquid or a solid, but this experiment only addressesthe determination of solids.

 JoVE Neuroscience

A Method for Systematic Electrochemical and Electrophysiological Evaluation of Neural Recording Electrodes

1School of Psychological Science, La Trobe University, 2Intelligent Polymer Research Institute, University of Wollongong, 3ARC Centre of Excellence for Electromaterials Science, 4Health Innovations Research Institute, College of Science, Engineering, and Health, RMIT University


JoVE 51084

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