JoVE Medicine serves as a connection between basic research and clinical applications. Articles may present medical procedures, case studies or clinical trial methodologies. Included studies are often modeled towards predictive, preventative, personalized, and practical approaches to treatment of disease. The goal of research methodologies in this section is to improve overall patient care and prognosis.
1Bloomberg School of Public Health, Environmental Health Sciences, Johns Hopkins University, 2Department of Medicine, Pulmonary Diseases and Critical Care Medicine, University of North Carolina School of Medicine
The goal of this paper is to describe simple methods that will greatly aid in the setup and analysis of mouse lungs with lung cancer or other pathologies. We present 3 protocols to simply and reliably carry out lung instillations, fixation, and lung volume measurements.
Published August 31, 2015. Keywords: Medicine, Lung histology, Lung morphometry, Lung fixation, Lung stereology, Lung volume, Lung Intubation
1Department of Thoracic Surgery, Ningxia Medical University General Hospital, 2Cancer Research Program, North Carolina Central University, 3Department of Thoracic & Cardiovascular Surgery, The First Affiliated Hospital of Chongqing Medical University, 4Department of Medicine, Center for Esophageal Disease and Swallowing, Division of Gastroenterology and Hepatology, University of North Carolina at Chapel Hill
This article demonstrates surgical procedures of gastroesophageal reflux with mice. These models are useful tools for research on mechanisms and treatment of gastroesophageal reflux disease and potentially Barrett’s esophagus and esophageal adenocarcinoma.
Published August 25, 2015. Keywords: Medicine, Gastroesophageal reflux disease, gastric reflux, mixed reflux, duodenal reflux, esophagus
1Department of Cardiovascular Surgery, Division of Experimental Surgery, German Heart Center Munich, Technische Universität München, 2Institute of Pathology, Helmholtz Zentrum Munich, 3Department of Cardiovascular Surgery, Division of Experimental Surgery, German Heart Center Munich, Technische Universität München, 4Department of Cardiovascular and Thoracic Surgery, General Hospital Linz
The cuff technique shortens and facilitates the model of heterotopic cervical heart transplantation in mice by avoiding technically challenging suture anastomoses of small vessels. The technical details shown in this video paper should allow researches to establish this model in their laboratories.
Published August 25, 2015. Keywords: Medicine, Heart, Transplantation, Mice, Cuff Technique, Heterotopic Heart Transplantation, Microsurgery, Ischemia-Reperfusion Injury
1Department of Pediatrics, Division of Neurology, The Emory University School of Medicine
Thromboembolic stroke models are vital tools for optimizing the recanalization therapy. Here we report a murine thrombotic stroke model based on transient cerebral hypoxic-ischemic (tHI) insult, which triggers thrombosis and infarction, and responds favorably to tissue plasminogen activator (tPA)-mediated fibrinolysis in a therapeutic window similar to those in stroke patients. …
Published August 18, 2015. Keywords: Medicine, Tissue plasminogen activator (tPA), Laser speckle contrast imaging, Thrombosis, Virchow’s triad, Edaravone, Reperfusion, No-Reflow
1Anatomy and Cell Biology, University of Kansas Medical Center
We are measuring perigenital mechanical sensitivity and mast cell activation in the prostate of male C57BL/6 mice that underwent an early life stress paradigm – neonatal maternal separation, in order to induce a preclinical model of chronic prostatitis/chronic pelvic pain syndrome.
Published August 13, 2015. Keywords: Medicine, Stress, chronic prostatitis, chronic pelvic pain, mice, mast cell, allodynia, hypothalamic-pituitary-adrenal axis
1Institute of Medical Sciences, University of Toronto, 2MRI-Guided rTMS Clinic, University Health Network, 3Department of Psychiatry, University Health Network, 4Toronto Western Research Institute, University Health Network, 5Department of Psychiatry, University of Toronto, 6Faculty of Arts and Science, University of Toronto, 7Temerty Centre for Therapeutic Brain Intervention, Centre for Addiction and Mental Health
Here we outline the procedure for MRI-guided repetitive transcranial magnetic stimulation to the dorsomedial prefrontal cortex as an experimental treatment for major depressive disorder.
Published August 11, 2015. Keywords: Medicine, Neuroscience, Magnetic Resonance Imaging (MRI) Guided, Repetitive Transcranial Magnetic Stimulation (rTMS), Dorsomedial Prefrontal Cortex (dmPFC), Major Depressive Disorder (MDD)
1Neurovascular and Stroke Center, Crouse Neuroscience Institute, 2Department of Biology, Syracuse University, 3Department of Neurosurgery, University of Florida, 4Department of Neurosurgery, SUNY Upstate Medical University
Minimally invasive thumb-sized pterional craniotomy for aneurysm clipping has afforded our patients with a shorter hospital stay at a lower cost compared to the national average.
Published August 11, 2015. Keywords: Medicine, craniotomy, cerebral aneurysm, clip ligation, health care costs, length of stay, minimally invasive, retractorless, surgery
1Diabetes Research Center, Vrije Universiteit Brussel
This protocol describes an injury model involving the surgical ligation of the pancreatic duct in the adult mouse pancreas, resulting in severe injury that establishes an environment that allows beta cell neogenesis and proliferation. This model can be used as a tool to study mechanisms involved in beta cell formation.
Published August 7, 2015. Keywords: Medicine, Pancreas, Partial duct ligation (PDL), injury, surgery, mouse model, cell differentiation, Neurogenin (Ngn) 3, cell reprogramming, cell proliferation.
1Department of Surgery, University of Michigan Medical School, 2Department of Biologic and Materials Sciences, University of Michigan School of Dentistry
An Achilles tenotomy and burn injury model of heterotopic ossification allows for the reliable study of trauma induced ectopic bone formation without the application of exogenous factors.
Published August 6, 2015. Keywords: Medicine, Heterotopic Ossification, Burn injury, Mouse model, Inflammation, µCT, Achilles tenotomy
1Cardiothoracic Surgery Research Center, Brown University, 2Rhode Island Hospital and Alpert Medical School, Brown University
The goal of this protocol to assess myocardial stunning following ischemic cardioplegic arrest in rodents.
Published August 6, 2015. Keywords: Medicine, Ischemia, Langendorff, cardioplegia, myocardial stunning, cardiac function, isolated hearts