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JoVE Medicine
JoVE Medicine serves as a connection between basic research and clinical applications. Articles may present medical procedures, case studies or clinical trial methodologies. Included studies are often modeled towards predictive, preventative, personalized, and practical approaches to treatment of disease. The goal of research methodologies in this section is to improve overall patient care and prognosis.
 JoVE Medicine

Ex Situ Normothermic Machine Perfusion of Donor Livers

1Section of Hepato-Pancreato-Biliary Surgery and Liver Transplantation, University of Groningen, University Medical Center Groningen, 2Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, 3Center of Engineering in Medicine/Surgical Services, Massachusetts General Hospital, Harvard Medical School, and Shriners Burns Hospital, 4Division of Transplantation, Department of Surgery, Massachusetts General Hospital, Harvard Medical School


JoVE 52688

Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.

 JoVE Medicine

Non-fluoroscopic Catheter Tracking for Fluoroscopy Reduction in Interventional Electrophysiology

1Department of Electrophysiology, Heart Center, University of Leipzig


JoVE 52606

Radiation exposure is an underestimated risk in complex ablation procedures. Here, we describe a protocol to significantly decrease fluoroscopy time and dosage for both the patient and the lab staff by using a novel non-fluoroscopic catheter visualization system.

 JoVE Medicine

Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography

1University/ BHF Centre for Cardiovascular Science, University of Edinburgh, The Queen's Medical Research Institute


JoVE 50627

Ex vivo analysis of arterial lesions from animal models of cardiovascular disease classically relies on histological and immunohistochemical techniques. These provide 2-dimensional measurements in 3-dimensional lesions. This manuscript describes the generation of arterial lesions for quantitative analysis in 3-dimensions using optical projection tomography.

 JoVE Medicine

Whole Vitreous Humor Dissection for Vitreodynamic Analysis

1Biomedical Engineering, University of Southern California, 2Ophthalmology, University of Southern California


JoVE 52759

The goal of this protocol is to show an effective technique to isolate whole, intact vitreous core and cortex from post mortem enucleated porcine eyes.

 JoVE Medicine

Use of Electromagnetic Navigational Transthoracic Needle Aspiration (E-TTNA) for Sampling of Lung Nodules

1Section of Interventional Pulmonology, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University


JoVE 52723

We describe the novel use of electromagnetic navigational guided transthoracic needle aspiration for the pathologic assessment of human lung nodules.

 JoVE Medicine

Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells

1Department of Baker Institute for Animal Health, Cornell University, 2Department of Molecular Biology and Genetics, Cornell University, 3Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School


JoVE 52727

Here, we document the use of the soft agar colony formation assay to test the effects of a peptidylarginine deiminase (PADI) enzyme inhibitor, BB-Cl-amidine, on breast cancer tumorigenicity in vitro.

 JoVE Medicine

Primary Tumor and MEF Cell Isolation to Study Lung Metastasis

1Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center


JoVE 52609

The goal of this protocol is to study breast tumorigenesis. With this technique, mouse mammary tumors are removed and primary cells are prepared from tumors. A lung extraction protocol is included for studying lung metastasis. Furthermore, another protocol for analyzing mouse embryonic fibroblasts from the mouse embryo is included.

 JoVE Medicine

Mouse Model of Alloimmune-induced Vascular Rejection and Transplant Arteriosclerosis

1Department of Molecular Biology and Biochemistry, Simon Fraser University


JoVE 52800

We describe a protocol for aortic interposition grafting in mice. The goal of the protocol is to provide a model with which to study pathological processes and therapeutic strategies relevant to alloimmune reactions in arteries and the resultant arterial changes that contribute to organ transplant failure.

 JoVE Medicine

A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy

1The Perinatal Research Unit, Department of Pediatrics, Institute of Clinical Medicine, Aarhus University Hospital, 2Department of Otorhinolaryngology and Head & Neck Surgery, Institute of Clinical Medicine, Aarhus University Hospital


JoVE 52454

Hypoxic-ischemic encephalopathy following perinatal asphyxia can be studied using animal models. We demonstrate the procedures necessary for establishing a piglet model of neonatal hypoxic-ischemic encephalopathy.

 JoVE Medicine

Do's and Don'ts in the Preparation of Muscle Cryosections for Histological Analysis

1Sargent College, Boston University


JoVE 52793

Here we demonstrate the most efficient methods for freezing, embedding, cryosectioning, and staining of muscle biopsies to avoid freezing artifacts.

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