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JoVE Medicine
JoVE Medicine serves as a connection between basic research and clinical applications. Articles may present medical procedures, case studies or clinical trial methodologies. Included studies are often modeled towards predictive, preventative, personalized, and practical approaches to treatment of disease. The goal of research methodologies in this section is to improve overall patient care and prognosis.
 JoVE Medicine

Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts

1Department of Medicine/Cardiology, Gazes Cardiac Research Institute, Medical University of South Carolina, 2Ralph H. Johnson VA Medical Center, Medical University of South Carolina


JoVE 52908

The isolated rat heart is an enduring model for ischemia reperfusion injury. Here, we describe the process of harvesting the beating heart from a rat via in situ aortic cannulation, Langendorff perfusion of the heart, simulated ischemia-reperfusion injury, and infarct staining to confirm the extent of ischemic insult.

 JoVE Medicine

Determining The Electromyographic Fatigue Threshold Following a Single Visit Exercise Test

1Physical Therapy Program and Integrative Physiology of Exercise Laboratory, Department of Health Care Sciences, Wayne State University, 2Sports Medicine and Physical Therapy, MEDSPORT, University of Michigan Health System, 3Department of Kinesiology, California State University, Fullerton


JoVE 52729

This protocol describes the electromyographic fatigue threshold which demarcates between nonfatiguing and fatiguing exercise workloads. This information could be used to develop a more individualized training program.

 JoVE Medicine

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes

1Department of Neurobiology and Anatomy, West Virginia University, 2Animal Models and Imaging Facility, West Virginia University, 3Mary Babb Randolph Cancer Center, West Virginia University


JoVE 52718

This protocol describes the application of high-frequency ultrasound (HFUS) for imaging mouse cervical lymph nodes. This technique optimizes visualization and quantification of cervical lymph node morphology, volume and blood flow. Image-guided biopsy of cervical lymph nodes and processing of lymph tissue for histological evaluation is also demonstrated.

 JoVE Medicine

Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells

1Department of Biology, Syracuse University


JoVE 53060

Selective damage of human leukemia cells can be achieved through a novel approach of applying low frequency ultrasound both with and without chemotherapeutic pretreatment of leukemic and normal hematopoietic cells.

 JoVE Medicine

Photothrombosis-induced Focal Ischemia as a Model of Spinal Cord Injury in Mice

1Department of Bioengineering, Dalton Cardiovascular Research Center, University of Missouri


JoVE 53161

Photothrombosis is a minimally invasive and highly reproducible procedure to induce focal ischemia in the spinal cord and serves as a model of spinal cord injury in mice.

 JoVE Medicine

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

1Department of Cancer Imaging and Metabolism, H. Lee Moffitt Cancer Center and Research Institute


JoVE 53070

We describe a high-throughput drug sensitivity assay for primary multiple myeloma cells. It consists of a reconstruction of the bone marrow microenvironment (including extracellular matrix and stroma) in multi-well plates, and a non-invasive method for longitudinal quantification of cell viability.

 JoVE Medicine

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation

1Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, 2Division of Nephrology, The Hospital for Sick Children, Toronto, 3Department of General, Visceral & Transplant Surgery, University Medical Center Mainz, 4Department of Abdominal, Vascular & Transplant Surgery, Merheim Medical Center Cologne, 5Laboratory Medicine & Pathobiology, Toronto General Hospital, 6Departments of Surgery (Urology) & Physiology, The Hospital for Sick Children, Toronto, 7Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto


JoVE 52909

The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to  transplantation.

 JoVE Medicine

An “All-laser” Endothelial Transplant

1Institute of Applied Physics, Italian National Research Council, 2U.O. Oculistica, Nuovo Ospedale Santo Stefano


JoVE 52939

An all laser procedure is proposed in the endothelial transplant. The surgical technique is based on the use of a femtosecond laser to prepare the donor tissue. Laser welding technique is then used to secure the donor endothelium in the correct position.

 JoVE Medicine

Therapeutic Effectiveness of a Dietary Supplement for Management of Halitosis in Dogs

1Department of Surgery and Surgical Specialties, Azienda Ospedaliero-Universitaria Policlinico di Modena, 2Department of Clinical Microbiology, Universtity of Modena and Reggio Emilia, 3Sanypet S.p.a, 4AIRMO Center Milan


JoVE 52717

We describe a simple approach for diagnosis of halitosis in dogs as well as a dietary approach for its management. This protocol may be extended to the management of halitosis in humans in the near future.

 JoVE Medicine

Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography

1Section of Gastroenterology and Hepatology, Georgia Regents University, 2Division of Gastroenterology and Hepatology, University of Pittsburgh School of Medicine, 3Vascular Biology Center, Georgia Regents University


JoVE 50997

Pressure myography is used to assess vasoactivity of small arteries that develop sustained constriction when pressurized. This manuscript provides a detailed protocol to assess in isolated segments of small mesenteric arteries from rats, vasoactivity and the effect of intraluminal pressure on vascular diameter.

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