Summary
यह पत्र एक साधारण मानव परिधीय रक्त कोशिकाओं mononuclear में alloantigen विशेष anergy प्रेरित तकनीक का वर्णन करता है. तकनीक चिकित्सकीय लागू किया जा सकता है गैर alloreactive दाता कोशिकाओं उत्पन्न. इन कोशिकाओं के आसव प्रतिरक्षा पुनर्गठन में सुधार और allogeneic hematopoietic स्टेम सेल प्रत्यारोपण के बाद विषाक्तता को कम कर सकता है.
Protocol
Discussion
Disclosures
ब्याज की कोई संघर्ष की घोषणा की.
Acknowledgments
Materials
Name | Company | Catalog Number | Comments |
Ficoll-Hypaque PLUS | GE Healthcare | 17-1440-02 | |
RPMI 1640 | Invitrogen | 11875-093 | |
Hepes 1M | Invitrogen | 15630-080 | |
L-Glutamine 200mM | Invitrogen | 25030-081 | |
Gentamicin | Invitrogen | 15750-060 | |
Human AB serum (heat inactivated) | Gemini Bio Products | 100-512 | Use validated batches |
Complete Culture Media (500ml) | 440ml RPMI 1640, 50ml AB serum, 5ml Hepes, 5 ml Glutamine, 167uL Gentamicin | ||
Irradiator | GammaCell 1000 | ||
T-25 Cell Culture Flasks with gas permeable caps | Corning | 3056 | |
Humanized Monoclonal anti-B7.1 and anti B7.2 antibodies | See protocol text | ||
U bottomed 96 well culture plates | BD Biosciences | 353077 | |
Monoclonal CD3 antibody | Beckman Coulter Inc. | IM0178 | Reconstitute with 200ml distilled water |
Monoclonal CD28 antibody | Beckman Coulter Inc. | IM1376 | Reconstitute with 200ml distilled water |
CMV grade 2 antigen | Microbix | EL-01-02 | |
Tritiated Thymidine | NEN | NET027 | |
Scintillation Fluid | PerkinElmer, Inc. | 1205-440 | |
Harvester | Tomtec | ||
Printed Filtermat A | PerkinElmer, Inc. | 1450-421 | |
Filter Bag | PerkinElmer, Inc. | 1450-432 | |
1450 MicroBeta TriLux Scintillation Counter | Wallac | ||
null |
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