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Journal / Cancer Research / 通过测量活细胞中的 eIF4E-eIF4G 交互来监控 eIF4F…
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Cancer Research

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JoVE Journal Cancer Research
通过测量活细胞中的 eIF4E-eIF4G 交互来监控 eIF4F 装配
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
Journal (Cancer Research)
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
Journal (Cancer Research)
Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
 
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通过测量活细胞中的 eIF4E-eIF4G 交互来监控 eIF4F 装配

Article DOI: 10.3791/60850-v • 08:47 min • May 1st, 2020
May 1st, 2020 •
, ,
1p53 Laboratory, Neuros/Immunos, A*STAR (Agency for Science, Technology and Research)

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在这里，我们提出了一个协议，以测量eIF4E-eIF4G在活细胞中的相互作用，使用户能够评估药物引起的扰动eIF4F复杂动态的筛选格式。

Tags

癌症研究，第159期，细胞生物学，eIF4F复合物，转化控制，路西法酶补充检测，mTOR信号，蛋白质-蛋白质相互作用
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