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Cancer Research

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JoVE Journal Cancer Research
Surveillance de l’assemblage eIF4F en mesurant l’interaction eIF4E-eIF4G dans les cellules vivantes
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Surveillance de l’assemblage eIF4F en mesurant l’interaction eIF4E-eIF4G dans les cellules vivantes

Article DOI: 10.3791/60850-v • 08:47 min • May 1st, 2020
May 1st, 2020 •
, ,
1p53 Laboratory, Neuros/Immunos, A*STAR (Agency for Science, Technology and Research)

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Ici, nous présentons un protocole pour mesurer l’interaction eIF4E-eIF4G dans les cellules vivantes qui permettrait à l’utilisateur d’évaluer la perturbation induite par les médicaments de la dynamique complexe eIF4F dans les formats de dépistage.

Tags

Recherche sur le cancer numéro 159 biologie cellulaire complexe eIF4F contrôle translationnel analyse de complémentarité luciferase signalisation mTOR interactions protéines-protéines
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