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Monitoring van eIF4F-assemblage door eIF4E-eIF4G-interactie in levende cellen te meten
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Monitoring van eIF4F-assemblage door eIF4E-eIF4G-interactie in levende cellen te meten

Article DOI: 10.3791/60850-v • 08:47 min • May 1st, 2020
May 1st, 2020 •
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1p53 Laboratory, Neuros/Immunos, A*STAR (Agency for Science, Technology and Research)

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Hier presenteren we een protocol om eIF4E-eIF4G-interactie in levende cellen te meten waarmee de gebruiker door geneesmiddelen veroorzaakte verstoring van de complexe dynamiek van eIF4F in screeningsformaten kan evalueren.

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Kankeronderzoek Nummer 159 Cellulaire biologie eIF4F-complex Translationele controle Luciferase complementatietest mTOR-signalering Eiwit-eiwitinteracties
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