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JoVE Journal Cancer Research
Forbedret levedygtighed for Ex vivo 3D Hydrogel Kulturer af patient-afledte Xenografts i en perfunderet microfluidic platform
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Forbedret levedygtighed for Ex vivo 3D Hydrogel Kulturer af patient-afledte Xenografts i en perfunderet microfluidic platform

Article DOI: 10.3791/60872-v • 12:12 min • December 5th, 2020
December 5th, 2020 •
1, 2, 3, 4, 1,5,6, 1,7, 2, 3, 1,5,6
1Department of BioSciences, Rice University, 2Mimetas US Inc, 3Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, 4Mimetas B.V., 5Department of Bioengineering, Rice University, 6Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center, 7Sheikh Ahmed Center for Pancreatic Cancer Research, The University of Texas MD Anderson Cancer Center

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Denne protokol demonstrerer metoder til at muliggøre udvidet in vitro-kultur af patientafledte xenografts (PDX). Et skridt øger den samlede levedygtighed af flercellede klyngekulturer i 3D-hydrogels ved simpel fjernelse af ikke-levedygtige enkeltceller. Et sekundært trin demonstrerer bedste praksis for PDX-kultur i en perfunderet mikrofluidisk platform.

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Cancer Research 3D kultur hydrogels microfluidics patient-afledte xenografts prostatakræft in vitro kræft modeller høj-throughput drug screening perfusion
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