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JoVE Journal Genetics
Avaliação quantitativa de PCR em tempo real de expressões de microRNA no rim do rato com obstrução ureteral unilateral
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Avaliação quantitativa de PCR em tempo real de expressões de microRNA no rim do rato com obstrução ureteral unilateral

Article DOI: 10.3791/61383-v • 07:36 min • August 27th, 2020
August 27th, 2020 •
1, 1, 1, 1,2, 1, 1, 1, 3, 1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

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Descrevemos um método para avaliar a expressão de microRNA nos rins de camundongos com obstrução ureteral unilateral (UUO) por reação quantitativa em cadeia de polimerase de transcrição reversa. Este protocolo é adequado para estudar perfis de expressão de microRNA renal em camundongos com UUO e no contexto de outras condições patológicas.

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Genética Edição 162 biologia molecular microRNA DNA complementar obstrução ureteral unilateral rim fibrose intersticial renal qRT-PCR
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