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Journal / Genetics / Quantitative Echtzeit-PCR-Bewertung von…
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JoVE Journal Genetics
Quantitative Echtzeit-PCR-Bewertung von microRNA-Expressions in der Mausniere mit einseitiger ureteraler Obstruktion
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Quantitative Echtzeit-PCR-Bewertung von microRNA-Expressions in der Mausniere mit einseitiger ureteraler Obstruktion

Article DOI: 10.3791/61383-v • 07:36 min • August 27th, 2020
August 27th, 2020 •
1, 1, 1, 1,2, 1, 1, 1, 3, 1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

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Wir beschreiben eine Methode zur Bewertung der microRNA-Expression in den Nieren von Mäusen mit einseitiger Harnleiterobstruktion (UUO) durch quantitative Reverse-Transkription-Polymerase-Kettenreaktion. Dieses Protokoll eignet sich zur Untersuchung von Nieren-MicroRNA-Expressionsprofilen bei Mäusen mit UUO und im Kontext anderer pathologischer Erkrankungen.

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Genetik Ausgabe 162 Molekularbiologie microRNA komplementäre DNA einseitige Harnleiterobstruktion Niere renale interstitielle Fibrose qRT-PCR
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