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JoVE Journal Genetics
Kvantitativ real-time PCR evaluering af microRNA udtryk i musenyr med ensidig ureteral obstruktion
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Kvantitativ real-time PCR evaluering af microRNA udtryk i musenyr med ensidig ureteral obstruktion

Article DOI: 10.3791/61383-v • 07:36 min • August 27th, 2020
August 27th, 2020 •
1, 1, 1, 1,2, 1, 1, 1, 3, 1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

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Vi beskriver en metode til vurdering af microRNA-ekspressionen i nyrerne af mus med ensidig urinlederobstruktion (UUO) ved kvantitativ omvendt transskription polymerase kædereaktion. Denne protokol er velegnet til undersøgelse af nyre microRNA-ekspressionsprofiler i mus med UUO og i forbindelse med andre patologiske tilstande.

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Genetik molekylærbiologi microRNA komplementær DNA ensidig urinlederobstruktion nyre nyre interstitiel fibrose qRT-PCR
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