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JoVE Journal Genetics
Kvantitativ Realtids PCR Utvärdering av mikroRNA-uttryck i Mus Njure med ensidig Ureteral obstruktion
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Kvantitativ Realtids PCR Utvärdering av mikroRNA-uttryck i Mus Njure med ensidig Ureteral obstruktion

Article DOI: 10.3791/61383-v • 07:36 min • August 27th, 2020
August 27th, 2020 •
1, 1, 1, 1,2, 1, 1, 1, 3, 1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

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Vi beskriver en metod för utvärdering av mikroRNA uttrycket i njurarna hos möss med ensidiga ureteral obstruktion (UUO) genom kvantitativa omvänd-transkription polymeras kedjereaktion. Detta protokoll är lämpligt för att studera njurmikroNARNA-uttrycksprofiler hos möss med UUO och i samband med andra patologiska tillstånd.

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Genetik molekylärbiologi mikroRNA komplementärt DNA ensidigt ureteral obstruktion njure njure njure interstitiell fibros qRT-PCR
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