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Developmental Biology

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JoVE Journal Developmental Biology
Differenzierung und Charakterisierung neuronaler Vorläufer und Neuronen aus embryonalen Stammzellen der Maus
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Differenzierung und Charakterisierung neuronaler Vorläufer und Neuronen aus embryonalen Stammzellen der Maus

Article DOI: 10.3791/61446-v • 08:47 min • May 15th, 2020
May 15th, 2020 •
1, 1, 1, 1
1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine, Penn State Hershey Cancer Institute

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Wir beschreiben das Verfahren zur In-vitro-Differenzierung von embryonalen Stammzellen der Maus in neuronale Zellen mit der Hanging-Drop-Methode. Darüber hinaus führen wir eine umfassende phänotypische Analyse durch RT-qPCR, Immunfluoreszenz, RNA-seq und Durchflusszytometrie durch.

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Developmental Biology Mouse embryonic stem cells embryoid bodies neural progenitor cell neurons differentiation hanging drop E14
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