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Developmental Biology

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JoVE Journal Developmental Biology
Differenziazione e caratterizzazione di progenitori neurali e neuroni da cellule staminali embrionali di topo
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Differenziazione e caratterizzazione di progenitori neurali e neuroni da cellule staminali embrionali di topo

Article DOI: 10.3791/61446-v • 08:47 min • May 15th, 2020
May 15th, 2020 •
1, 1, 1, 1
1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine, Penn State Hershey Cancer Institute

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Descriviamo la procedura per la differenziazione in vitro di cellule staminali embrionali di topo in cellule neuronali utilizzando il metodo della goccia sospesa. Inoltre, eseguiamo un'analisi fenotipica completa attraverso RT-qPCR, immunofluorescenza, RNA-seq e citometria a flusso.

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Biologia dello sviluppo Numero 159 Cellule staminali embrionali di topo corpi embrioidi cellula progenitrice neurale neuroni differenziazione goccia sospesa E14
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