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Journal / Developmental Biology / マウス胚性幹細胞からの神経前駆細胞とニューロンの分化と特徴付け…
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Developmental Biology

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JoVE Journal Developmental Biology
マウス胚性幹細胞からの神経前駆細胞とニューロンの分化と特徴付け
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
Journal (Developmental Biology)
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
Journal (Developmental Biology)
Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
 
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マウス胚性幹細胞からの神経前駆細胞とニューロンの分化と特徴付け

Article DOI: 10.3791/61446-v • 08:47 min • May 15th, 2020
May 15th, 2020 •
1, 1, 1, 1
1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine, Penn State Hershey Cancer Institute

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吊り下げドロップ法を用いて、マウス胚性幹細胞を神経細胞に分化する手順について説明する。また、RT-qPCR、免疫蛍光、RNA-seq、フローサイトメトリーを通して包括的なフェノミック解析を行っています。

Tags

発生生物学、課題159、マウス胚性幹細胞、胚体、神経前駆細胞、ニューロン、分化、吊り下げドロップ、E14
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