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Journal / Biochemistry / Cellefraksjonering av U937-celler ved isopycnic…
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Biochemistry

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JoVE Journal Biochemistry
Cellefraksjonering av U937-celler ved isopycnic tetthetsgradientrensing
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Cellefraksjonering av U937-celler ved isopycnic tetthetsgradientrensing

Article DOI: 10.3791/62119-v • 10:19 min • August 12th, 2021
August 12th, 2021 •
1, 1, 1, 1, 1
1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences

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Denne fraksjoneringsprotokollen vil tillate forskere å isolere cytoplasmatiske, kjernefysiske, mitokondrielle og membranproteiner fra pattedyrceller. De to sistnevnte subcellulære fraksjonene blir videre renset via isopycnic tetthetsgradient.

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Biokjemi utgave 174 Cellulær fraksjonering Nuclear Mitochondria Membran Cytoplasma Organeller Ultracentrifugation Density Gradient U937
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