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Journal / Genetics / Tillämpning av CRISPR-interferens (CRISPRi) för…
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Genetics
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JoVE Journal Genetics
Tillämpning av CRISPR-interferens (CRISPRi) för gensilencing hos patogena arter av Leptospira
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Tillämpning av CRISPR-interferens (CRISPRi) för gensilencing hos patogena arter av Leptospira

Article DOI: 10.3791/62631-v • 14:49 min • August 14th, 2021
August 14th, 2021 •
1,2, 1, *2, *1
1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2Laboratório de Desenvolvimento de Vacinas, Instituto Butantan
* These authors contributed equally

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Här beskrivs tillämpningen av CRISPR-interferens (CRISPRi) för specifik gensilencing i Leptospira arter. Leptospiraceller omvandlas genom konjugation med plasmider som uttrycker dCas9 (katalytiskt "döda" Cas9) och en enguide RNA (sgRNA), ansvarig för basparning till önskat genomiskt mål. Metoder för att validera gensilencing presenteras.

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Genetik Utgåva 174 Leptospira leptospiros mutagenes CRISPR-interferens gensilencing konjugation
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