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JoVE Journal Genetics
Påføring av CRISPR Interference (CRISPRi) for genaktivering i patogene arter av leptospira
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Påføring av CRISPR Interference (CRISPRi) for genaktivering i patogene arter av leptospira

Article DOI: 10.3791/62631-v • 14:49 min • August 14th, 2021
August 14th, 2021 •
1,2, 1, *2, *1
1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2Laboratório de Desenvolvimento de Vacinas, Instituto Butantan
* These authors contributed equally

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Her beskrives anvendelsen av CRISPR-interferens (CRISPRi) for spesifikk genaktivering i Leptospira-arter. Leptospira celler er forvandlet ved konjugering med plasmider som uttrykker dCas9 (katalytisk "død" Cas9) og en enkelt guide RNA (sgRNA), ansvarlig for base paring til ønsket genomisk mål. Metoder for å validere genaktivering presenteres.

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Genetikk Utgave 174 Leptospira leptospirose mutagenese CRISPR-interferens genaktivering konjugering
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