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Journal / Immunology and Infection / 미세주입에 의한 스트 롱실로이드 종에서 형질전환 및 녹아웃…
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Immunology and Infection

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JoVE Journal Immunology and Infection
미세주입에 의한 스트 롱실로이드 종에서 형질전환 및 녹아웃 생성
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Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models
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미세주입에 의한 스트 롱실로이드 종에서 형질전환 및 녹아웃 생성

Article DOI: 10.3791/63023-v • 09:42 min • October 7th, 2021
October 7th, 2021 •
1, 1,2
1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles

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기생 선충류 Strongyloides stercoralis 및 Strongyloides ratti 에 대한 기능적 게놈 툴킷에는 트랜스제네시스, CRISPR/Cas9 매개 돌연변이유발 및 RNAi가 포함됩니다. 이 프로토콜은 성선 내 미세주사를 사용하여 전이유전자 및 CRISPR 성분을 S. stercoralis 및 S. ratti에 도입하는 방법을 입증할 것이다.

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면역학 및 감염 문제 176
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