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Immunology and Infection

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JoVE Journal Immunology and Infection
Generera transgenik och knockouts i Strongyloides-arter genom mikroinjektion
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Generera transgenik och knockouts i Strongyloides-arter genom mikroinjektion

Article DOI: 10.3791/63023-v • 09:42 min • October 7th, 2021
October 7th, 2021 •
1, 1,2
1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles

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Den funktionella genomiska verktygslådan för parasitiska nematoder Strongyloides stercoralis och Strongyloides ratti inkluderar transgenes, CRISPR/ Cas9-medierad mutagenes och RNAi. Detta protokoll kommer att visa hur man använder intragonad mikroinjektion för att införa transgener och CRISPR-komponenter i S. stercoralis och S. ratti.

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Immunologi och infektion utgåva 176
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