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Journal / Immunology and Infection / Strongyloides Türlerinde Mikroenjeksiyon ile…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Strongyloides Türlerinde Mikroenjeksiyon ile Transgenik ve Nakavt Üretimi
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Strongyloides Türlerinde Mikroenjeksiyon ile Transgenik ve Nakavt Üretimi

Article DOI: 10.3791/63023-v • 09:42 min • October 7th, 2021
October 7th, 2021 •
1, 1,2
1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles

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Parazitik nematodlar Strongyloides stercoralis ve Strongyloides ratti için fonksiyonel genomik araç seti transgenez, CRISPR / Cas9 aracılı mutagenez ve RNAi'yi içerir. Bu protokol, transgenleri ve CRISPR bileşenlerini S. stercoralis ve S . ratti'ye tanıtmak için intragonadal mikroenjeksiyonun nasıl kullanılacağını gösterecektir.

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