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Immunology and Infection

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JoVE Journal Immunology and Infection
Erzeugung von Transgenics und Knockouts in Strongyloides-Spezies durch Mikroinjektion
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Erzeugung von Transgenics und Knockouts in Strongyloides-Spezies durch Mikroinjektion

Article DOI: 10.3791/63023-v • 09:42 min • October 7th, 2021
October 7th, 2021 •
1, 1,2
1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles

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Das funktionelle genomische Toolkit für die parasitären Nematoden Strongyloides stercoralis und Strongyloides ratti umfasst Transgenese, CRISPR/Cas9-vermittelte Mutagenese und RNAi. Dieses Protokoll wird zeigen, wie intragonadale Mikroinjektion verwendet werden kann, um Transgene und CRISPR-Komponenten in S. stercoralis und S. ratti einzuführen.

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Immunologie und Infektion Ausgabe 176
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