Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / 尿嘧啶-DNA糖基化酶测定，采用基质辅助激光解吸/电离飞行时间质谱分析…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biochemistry
尿嘧啶-DNA糖基化酶测定，采用基质辅助激光解吸/电离飞行时间质谱分析
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
Journal (Biochemistry)
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
Journal (Biochemistry)
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
 
Click here for the English version

尿嘧啶-DNA糖基化酶测定，采用基质辅助激光解吸/电离飞行时间质谱分析

Article DOI: 10.3791/63089-v • 12:19 min • April 22nd, 2022
April 22nd, 2022 •
*1, *1,2, 3, 2, 1,2, 1,2, 1,2, 1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics, The Ohio State University
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

使用MALDI-TOF质谱法开发了一种非标记，非放射性同位素方法来测定尿嘧啶-DNA糖基化酶活性，用于直接分析含阳离子/嘧啶位点的产品。该测定被证明是非常简单，特异性，快速且易于使用的DNA糖基化酶测量。

Tags

生物化学，第182期，碱基切除修复，MALDI-TOF质谱，尿嘧啶-DNA糖基化酶，apurinic/嘧啶位点，糖基化酶抑制剂
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter