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Journal / Biochemistry / Uracil-DNA-Glykosylase-Assay durch…
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Biochemistry

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JoVE Journal Biochemistry
Uracil-DNA-Glykosylase-Assay durch matrixgestützte Laserdesorption/Ionisations-Time-of-Flight-Massenspektrometrie-Analyse
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Uracil-DNA-Glykosylase-Assay durch matrixgestützte Laserdesorption/Ionisations-Time-of-Flight-Massenspektrometrie-Analyse

Article DOI: 10.3791/63089-v • 12:19 min • April 22nd, 2022
April 22nd, 2022 •
*1, *1,2, 3, 2, 1,2, 1,2, 1,2, 1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics, The Ohio State University
* These authors contributed equally

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Eine nicht markierte, nicht-radioisotopische Methode zur Bestimmung der Uracil-DNA-Glykosylase-Aktivität wurde unter Verwendung der MALDI-TOF-Massenspektrometrie für die direkte apurinische/apyrimidinische standorthaltige Produktanalyse entwickelt. Der Assay erwies sich als ziemlich einfach, spezifisch, schnell und leicht zu bedienen für die DNA-Glykosylase-Messung.

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Biochemie Ausgabe 182 Base excision repair MALDI-TOF-Massenspektrometrie Uracil-DNA-Glykosylase apurinische/apyrimidinische Stelle Glykosylase-Inhibitor
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