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Journal / Biochemistry / Uracil-DNA Glykosylasanalys av Matrix-assisterad…
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Biochemistry

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JoVE Journal Biochemistry
Uracil-DNA Glykosylasanalys av Matrix-assisterad laserdesorption/joniseringStid för flygning Masspektrometrianalys
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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Journal (Biochemistry)
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
 
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Uracil-DNA Glykosylasanalys av Matrix-assisterad laserdesorption/joniseringStid för flygning Masspektrometrianalys

Article DOI: 10.3791/63089-v • 12:19 min • April 22nd, 2022
April 22nd, 2022 •
*1, *1,2, 3, 2, 1,2, 1,2, 1,2, 1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics, The Ohio State University
* These authors contributed equally

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En icke-märkt, icke-radio-isotopisk metod för att analysera uracil-DNA glykosylas aktivitet utvecklades med maldi-TOF masspektrometri för direkt apurinic/apyrimidinic platsinnehållande produktanalys. Analysen visade sig vara ganska enkel, specifik, snabb och lätt att använda för DNA-glykosylasmätning.

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Biokemi nummer 182 Basexcision reparation MALDI-TOF masspektrometri Uracil-DNA glykosylas apurinisk/apyrimidinisk plats glykosylashämmare
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