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Live-3D-cel immunocytochemie Assays van pediatrische diffuse middellijn glioom

Article DOI: 10.3791/63091-v • 09:06 min • November 11th, 2021
November 11th, 2021 •
*1, *1, 2,3, 1
1Department of Onco-hematology, Gene and Cell Therapy, Bambino Gesù Children's Hospital, IRCCS, 2Oncology Service, Children's Health Queensland Hospital and Health Service, 3Child Health Research Centre, The University of Queensland
* These authors contributed equally

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Deze studie presenteert een protocol van live-3D-cel immunocytochemie toegepast op een pediatrische diffuse midline glioom cellijnlijn, nuttig om in real-time de expressie van eiwitten op het plasmamembraan te bestuderen tijdens dynamische processen zoals 3D-celinvasie en migratie.

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Deze maand in JoVE nummer 177
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