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Ensaios de imunocitoquímica de células 3D vivas de glioma difuso da linha média pediátrica

Article DOI: 10.3791/63091-v • 09:06 min • November 11th, 2021
November 11th, 2021 •
*1, *1, 2,3, 1
1Department of Onco-hematology, Gene and Cell Therapy, Bambino Gesù Children's Hospital, IRCCS, 2Oncology Service, Children's Health Queensland Hospital and Health Service, 3Child Health Research Centre, The University of Queensland
* These authors contributed equally

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Este estudo apresenta um protocolo de imunocitoquímica de células vivas 3D aplicado a uma linhagem celular pediátrica de glioma difuso da linha média, útil para estudar em tempo real a expressão de proteínas na membrana plasmática durante processos dinâmicos como invasão e migração de células 3D.

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Este mês no JoVE edição 177
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