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Ensayos de inmunocitoquímica de células 3D vivas del glioma difuso de línea media infantil

Article DOI: 10.3791/63091-v • 09:06 min • November 11th, 2021
November 11th, 2021 •
*1, *1, 2,3, 1
1Department of Onco-hematology, Gene and Cell Therapy, Bambino Gesù Children's Hospital, IRCCS, 2Oncology Service, Children's Health Queensland Hospital and Health Service, 3Child Health Research Centre, The University of Queensland
* These authors contributed equally

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Este estudio presenta un protocolo de inmunocitoquímica de células vivas en 3D aplicado a una línea celular de glioma difuso de línea media pediátrico, útil para estudiar en tiempo real la expresión de proteínas en la membrana plasmática durante procesos dinámicos como la invasión y migración de células 3D.

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Este mes en JoVE número 177
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