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Immunology and Infection

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JoVE Journal Immunology and Infection

인간 단핵구 유래 대식세포에서 근접성을 유도한 염증성 카스파제의 시각화

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Journal (Immunology and Infection)

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

Journal (Immunology and Infection)

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

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인간 단핵구 유래 대식세포에서 근접성을 유도한 염증성 카스파제의 시각화

Article DOI: 10.3791/63162-v • 08:41 min • April 6th, 2022

April 6th, 2022 •

Beatriz E. Bolívar¹, Lisa Bouchier-Hayes¹

¹Department of Pediatrics, Division of Hematology-Oncology, Department of Molecular and Cellular Biology, Texas Children’s Hospital William T. Shearer Center for Human Immunobiology, Baylor College of Medicine

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이 프로토콜은 인간 혈액 샘플로부터 단핵구 유래 대식세포 (MDM)를 얻는 워크 플로우, 세포 생존력과 행동을 손상시키지 않고 염증성 카스파제 BiFC (BiFC) 리포터를 인간 MDM에 효율적으로 도입하는 간단한 방법, 살아있는 세포에서 염증성 카스파제 활성화를 측정하기위한 이미징 기반 접근법을 설명합니다.

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