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Journal / Biochemistry / E3泛素连接酶在哺乳动物细胞裂解物中底物泛素化的评价…
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Biochemistry

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JoVE Journal Biochemistry
E3泛素连接酶在哺乳动物细胞裂解物中底物泛素化的评价
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Journal (Biochemistry)
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E3泛素连接酶在哺乳动物细胞裂解物中底物泛素化的评价

Article DOI: 10.3791/63561-v • 09:47 min • May 10th, 2022
May 10th, 2022 •
*1, *2, 1, 1,2
1Department of Genetic and Evolution, Federal University of São Carlos, 2Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo
* These authors contributed equally

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我们为哺乳动物细胞中特定底物和E3泛素连接酶的泛素化测定提供了详细的方案。HEK293T细胞系用于蛋白质过表达，通过免疫沉淀从细胞裂解物中纯化多泛型底物，并在SDS-PAGE中分离。免疫印迹用于可视化这种翻译后修饰。

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生物化学，第183期，
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