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Journal / Biochemistry / Utvärdering av substrat Ubiquitylation av E3…
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Biochemistry

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JoVE Journal Biochemistry
Utvärdering av substrat Ubiquitylation av E3 Ubiquitin-ligas i däggdjurscelllysater
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Journal (Biochemistry)
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Utvärdering av substrat Ubiquitylation av E3 Ubiquitin-ligas i däggdjurscelllysater

Article DOI: 10.3791/63561-v • 09:47 min • May 10th, 2022
May 10th, 2022 •
*1, *2, 1, 1,2
1Department of Genetic and Evolution, Federal University of São Carlos, 2Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo
* These authors contributed equally

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Vi tillhandahåller ett detaljerat protokoll för en ubiquitylationsanalys av ett specifikt substrat och en E3 ubiquitin-ligas i däggdjursceller. HEK293T-cellinjer användes för proteinöveruttryck, det polyubiquitylerade substratet renades från celllysater genom immunoprecipitation och löstes i SDS-PAGE. Immunoblotting användes för att visualisera denna post-translationella modifiering.

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Biokemi utgåva 183
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