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Journal / Biochemistry / Single-Molecule Analyse van Sf9 Purified…
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JoVE Journal Biochemistry
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Single-Molecule Analyse van Sf9 Purified Superprocessive Kinesin-3 Family Motoren

Article DOI: 10.3791/63837-v • 08:16 min • July 27th, 2022
July 27th, 2022 •
1,2, 1, 2, 1
1Discipline of Biological Engineering, Indian Institute of Technology Gandhinagar, 2Department of Biotechnology and Bioinformatics, Sambalpur University

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Deze studie beschrijft de zuivering van KIF1A (1-393LZ), een lid van de kinesine-3-familie, met behulp van het Sf9-baculovirusexpressiesysteem. In vitro single-molecule en multi-motor zweefanalyse van deze gezuiverde motoren vertoonde robuuste motiliteitseigenschappen vergelijkbaar met motoren van zoogdiercellysaat. Sf9-baculovirussysteem is dus vatbaar voor expressie en zuivering van motoreiwit van belang.

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Biochemie nummer 185
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