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Journal / Developmental Biology / 受精卵显微注射用于在小鼠中产生基因盒敲入和絮状等位基因…
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Developmental Biology

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JoVE Journal Developmental Biology
受精卵显微注射用于在小鼠中产生基因盒敲入和絮状等位基因
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受精卵显微注射用于在小鼠中产生基因盒敲入和絮状等位基因

Article DOI: 10.3791/64161-v • 08:48 min • June 24th, 2022
June 24th, 2022 •
*1, *1,2, 1, 1, 1, 1, 1, 1
1Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba
* These authors contributed equally

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本协议描述了CRISPR-Cas9和供体DNA的受精卵显微注射，以有效地产生基因盒敲入和絮凝小鼠。

Tags

发育生物学，第184期，CRISPR-Cas9，基因组编辑，转基因小鼠，基因盒敲入，flox，受精卵显微注射，压电
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