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Developmental Biology

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JoVE Journal Developmental Biology
Microinjeção de zigoto para criação de alelos knock-in e flox de gênico em camundongos
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Microinjeção de zigoto para criação de alelos knock-in e flox de gênico em camundongos

Article DOI: 10.3791/64161-v • 08:48 min • June 24th, 2022
June 24th, 2022 •
*1, *1,2, 1, 1, 1, 1, 1, 1
1Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba
* These authors contributed equally

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O presente protocolo descreve a microinjeção de zigoto de CRISPR-Cas9 e DNA do doador para produzir eficientemente camundongos knock-in e floxed de gênico.

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Biologia do Desenvolvimento Edição 184 CRISPR-Cas9 edição do genoma camundongo geneticamente modificado knock-in de genético flox microinjeção de zigoto piezo
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