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Journal / Developmental Biology / Micro-injection de zygote pour la création…
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Developmental Biology

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Micro-injection de zygote pour la création d’allèles knock-in et flox de cassette génique chez la souris
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Micro-injection de zygote pour la création d’allèles knock-in et flox de cassette génique chez la souris

Article DOI: 10.3791/64161-v • 08:48 min • June 24th, 2022
June 24th, 2022 •
*1, *1,2, 1, 1, 1, 1, 1, 1
1Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba
* These authors contributed equally

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Le présent protocole décrit la micro-injection de zygotes de CRISPR-Cas9 et d’ADN de donneur pour produire efficacement des souris frappées par cassette de gènes et floxées.

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Developmental Biology CRISPR-Cas9 édition du génome souris génétiquement modifiée cassette génétique knock-in flox micro-injection de zygote piézo
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