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JoVE Journal Bioengineering
Gene digitale circuits op basis van CRISPR-Cas-systemen en anti-CRISPR-eiwitten
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Gene digitale circuits op basis van CRISPR-Cas-systemen en anti-CRISPR-eiwitten

Article DOI: 10.3791/64539-v • 10:46 min • October 18th, 2022
October 18th, 2022 •
*1, *1, 1
1School of Pharmaceutical Science and Technology, Tianjin University
* These authors contributed equally

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CRISPR-Cas-systemen en anti-CRISPR-eiwitten werden geïntegreerd in het schema van Booleaanse poorten in Saccharomyces cerevisiae. De nieuwe kleine logische circuits toonden goede prestaties en verdiepten het begrip van zowel op dCas9/dCas12a gebaseerde transcriptiefactoren als de eigenschappen van anti-CRISPR-eiwitten.

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Retractie CRISPR-Cas-systeem dSpCas9 denAsCas12a dLbCas12a type II-A anti-CRISPR-eiwitten type V-A anti-CRISPR-eiwitten gendigitale circuits Saccharomyces cerevisiae synthetische biologie
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