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Journal / Bioengineering / Circuitos Digitais Gênicos Baseados em Sistemas…
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Bioengineering

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JoVE Journal Bioengineering
Circuitos Digitais Gênicos Baseados em Sistemas CRISPR-Cas e Proteínas Anti-CRISPR
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Circuitos Digitais Gênicos Baseados em Sistemas CRISPR-Cas e Proteínas Anti-CRISPR

Article DOI: 10.3791/64539-v • 10:46 min • October 18th, 2022
October 18th, 2022 •
*1, *1, 1
1School of Pharmaceutical Science and Technology, Tianjin University
* These authors contributed equally

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Sistemas CRISPR-Cas e proteínas anti-CRISPR foram integrados ao esquema de portões booleanos em Saccharomyces cerevisiae. Os novos circuitos lógicos pequenos mostraram bom desempenho e aprofundaram a compreensão dos fatores de transcrição baseados em dCas9/dCas12a e das propriedades das proteínas anti-CRISPR.

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Retração sistema CRISPR-Cas dSpCas9 denAsCas12a dLbCas12a proteínas anti-CRISPR tipo II-A proteínas anti-CRISPR tipo V-A circuitos digitais de genes Saccharomyces cerevisiae biologia sintética
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