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JoVE Journal Cancer Research
Modellazione del carcinoma a cellule squamose orale-esofagea in organoidi 3D
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Modellazione del carcinoma a cellule squamose orale-esofagea in organoidi 3D

Article DOI: 10.3791/64676-v • 10:43 min • December 23rd, 2022
December 23rd, 2022 •
*1, *1,2, 1, 1, 1, 1, 1, 1,3, 1,3, 4, 1,2, 1,5, 1,6, 1,7, 1,5, 1,2,5
1Herbert Irving Comprehensive Cancer Center, Columbia University, 2Organoid and Cell Culture Core, Columbia University Digestive and Liver Diseases Research Center, Columbia University, 3Department of Otolaryngology, Head and Neck Surgery, Columbia University, 4Histopathology Facility, Fox Chase Cancer Center, 5Division of Digestive and Liver Diseases, Department of Medicine, Columbia University, 6Department of Genetics and Development, Columbia University, 7Section of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine, Columbia University
* These authors contributed equally

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Questo protocollo descrive i passaggi chiave per generare e caratterizzare organoidi 3D orali-esofagei murini che rappresentano lesioni da carcinoma a cellule squamose normali, preneoplastiche e squamose indotte tramite carcinogenesi chimica.

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