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Modelagem do carcinoma epidermóide orco-esofágico em organoides 3D
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Modelagem do carcinoma epidermóide orco-esofágico em organoides 3D

Article DOI: 10.3791/64676-v • 10:43 min • December 23rd, 2022
December 23rd, 2022 •
*1, *1,2, 1, 1, 1, 1, 1, 1,3, 1,3, 4, 1,2, 1,5, 1,6, 1,7, 1,5, 1,2,5
1Herbert Irving Comprehensive Cancer Center, Columbia University, 2Organoid and Cell Culture Core, Columbia University Digestive and Liver Diseases Research Center, Columbia University, 3Department of Otolaryngology, Head and Neck Surgery, Columbia University, 4Histopathology Facility, Fox Chase Cancer Center, 5Division of Digestive and Liver Diseases, Department of Medicine, Columbia University, 6Department of Genetics and Development, Columbia University, 7Section of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine, Columbia University
* These authors contributed equally

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Este protocolo descreve os principais passos para gerar e caracterizar organoides 3D oro-esofágicos murinos que representam lesões normais, pré-neoplásicas e de carcinoma espinocelular induzidas por carcinogênese química.

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Pesquisa sobre o Câncer Edição 190
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