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JoVE Journal Biology
Celspecifieke gepaarde ondervraging van het ovarium-epigenoom en transcriptoom van de muis
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Celspecifieke gepaarde ondervraging van het ovarium-epigenoom en transcriptoom van de muis

Article DOI: 10.3791/64765-v • 12:25 min • February 24th, 2023
February 24th, 2023 •
*1,2,3, *1,3, 1, 2, 2, 4, 2,3, 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
* These authors contributed equally

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In dit protocol werden de translating ribosome affinity purification (TRAP) methode en de isolatie van nuclei tagged in specific cell types (INTACT) methode geoptimaliseerd voor de gepaarde ondervraging van het celspecifieke ovariumtranscriptoom en epigenoom met behulp van het NuTRAP muismodel gekruist naar een Cyp17a1-Cre muislijn.

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Deze maand in JoVE nummer 192
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