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Interrogación pareada específica de células del epigenoma ovárico y transcriptoma de ratón
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Interrogación pareada específica de células del epigenoma ovárico y transcriptoma de ratón

Article DOI: 10.3791/64765-v • 12:25 min • February 24th, 2023
February 24th, 2023 •
*1,2,3, *1,3, 1, 2, 2, 4, 2,3, 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
* These authors contributed equally

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En este protocolo, el método de purificación de afinidad ribosómica por traducción (TRAP) y el método de aislamiento de núcleos marcados en tipos celulares específicos (INTACT) se optimizaron para la interrogación pareada del transcriptoma ovárico específico de células y el epigenoma utilizando el modelo de ratón NuTRAP cruzado a una línea de ratón Cyp17a1-Cre.

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