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Journal / Biology / Fare yumurtalık epigenomu ve transkriptomunun…
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Fare yumurtalık epigenomu ve transkriptomunun hücreye özgü eşleştirilmiş sorgulaması
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Fare yumurtalık epigenomu ve transkriptomunun hücreye özgü eşleştirilmiş sorgulaması

Article DOI: 10.3791/64765-v • 12:25 min • February 24th, 2023
February 24th, 2023 •
*1,2,3, *1,3, 1, 2, 2, 4, 2,3, 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
* These authors contributed equally

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Bu protokolde, çevirici ribozom afinite saflaştırma (TRAP) yöntemi ve spesifik hücre tiplerinde etiketlenmiş çekirdeklerin izolasyonu (INTACT) yöntemi, bir Cyp17a1-Cre fare hattına geçilen NuTRAP fare modeli kullanılarak hücreye özgü yumurtalık transkriptomunun ve epigenomun eşleştirilmiş sorgulaması için optimize edilmiştir.

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