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Cellespecifik parret forhør af musens ovarieepigenom og transkriptom

Article DOI: 10.3791/64765-v • 12:25 min • February 24th, 2023
February 24th, 2023 •
*1,2,3, *1,3, 1, 2, 2, 4, 2,3, 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
* These authors contributed equally

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I denne protokol blev metoden til oversættelse af ribosomaffinitetsrensning (TRAP) og isolering af kerner mærket i specifikke celletyper (INTACT) optimeret til parret forhør af det cellespecifikke ovarietranskriptom og epigenom ved hjælp af NuTRAP-musemodellen krydset til en Cyp17a1-Cre-muselinje.

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Denne måned i JoVE nummer 192
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