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Journal / Bioengineering / Imagem Química Bioortogonal do Metabolismo…
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JoVE Journal Bioengineering
Imagem Química Bioortogonal do Metabolismo Celular Regulado por Aminoácidos Aromáticos
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Imagem Química Bioortogonal do Metabolismo Celular Regulado por Aminoácidos Aromáticos

Article DOI: 10.3791/65121-v • 10:42 min • May 12th, 2023
May 12th, 2023 •
*1, *1, 1, 1, 1, 1
1Shu Chien-Gene Lay Department of Bioengineering, University of California San Diego
* These authors contributed equally

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Apresentamos um protocolo para visualizar diretamente as atividades metabólicas em células reguladas por aminoácidos usando microscopia Raman estimulada de espalhamento Raman estimulado com óxido de deutério (água pesada D2O) (DO-SRS), que é integrada à microscopia de fluorescência por excitação de dois fótons (2PEF).

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Bioengenharia Edição 195
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